Background: The aim of study is to formulate and assess the herbal mouthwash that possesses anti-inflammatory properties and is used in the treatment of periodontal disease. The chief ingredient of this formulation is propolis or bee glue obtained from the honeycomb.

Method: To assess the quality of the mouthwash, organoleptic features such as color, odor and appearance were evaluated. Chemical and physical characteristics such as pH, viscosity, bio burden, and irritancy test on the skin was carried out. Mouthwash was tested in the accelerated stability condition along with 40 °C / 75 % relative humidity and at room temperature (RT) for 6 months. At the end of the trial, participants were interviewed about the different aspects and outcome of the mouthwash to assess the level of acceptance by the participants.

Result: The resultant formulation is clear, brown and sweet. The final pH and viscosity of the mouthwash is 5.0 and 630cp respectively and none of the physical properties were altered at accelerated statibility temperatures.

Conclusion: The mouthwash is effective in the treatment of periodontitis and the composition is stable in accelerated stability conditions upto 6 months. No sign of irritation or bacterial growth is seen. When interviewed, participants responded with satisfactory remarks.

 Keywords- Mouthwash, Propolis, Periodontal disease, Stability studies.