Objective: In-vitro anthelmintic activity of mother tincture of Artemisia cina, Trachyspermum ammi and Punica granatum were investigated on the eggs and adult nematodes of Haemonchus contortus.

Methodology:In vitro, eggs were exposed to different doses (75, 150, 300, 600, 1200, and 2400 µL) of mother tincture while adult Haemonchus contortus were exposed to different doses (250, 500, 1000, 2000, 4000, and 8000µL)following the standard procedures of egg hatch test and adult motility assay.Standard drug (Albendazole) 0.5mg/ml was used as a reference drug and normal saline as a control group.

Results:Percentage motility inhibition for Artemisia cina, Trachyspermum ammi andPunica granatum were observed on highest dose (8000 µL) as 81. 44%, 70.33% and 62.89%  respectively whilepercentage hatch inhibition for Artemisia cina, Trachyspermum ammi andPunica granatum were also observed onhighest dose (2400 µL) as 76.85%, 70.48% and 69.82% respectively. The most potent homeopathic mother tincture of plants inhibiting egg hatching based on LC50 was Artemisia cina (LC₅₀=247.6281 µL) followed by Punica granatum (LC₅₀=364.8879µL) and Trachyspermum ammi (LC₅₀=610.942µL) while the most potent homeopathic mother tincture of plants inhibiting motility of adult worms based on LC50 was Artemisia cina (LC₅₀=1601.1052µL) followed by Punica granatum (LC₅₀= 2463.9619µL) and Trachyspermum ammi (LC₅₀= 3210.1180µL). The data of correlation of regression revealed the best dose-dependent effects on egg hatching with Trachyspermum ammi (R² = 0.9936) followed by Punica granatum (R² =0.9759) and Artemisia cina (R² = 0.9529) while on motility of worms with Trachyspermum ammi (R² = 0.973) followed by Artemisia cina (R² = 0.9970)and Punica granatum (R² =0.855).

Conclusion:The result therefore, showed that these homeopathic medicines(Cina)Artemisia cina, (T. ammi)Trachyspermum ammi and(Granatum)Punica granatum have therapeutic option to target adult worms and to prevent eggs from hatching, reduce the excretion and avoid environmental contamination. However, further experimentation is needed in this regard to decipher this activity.

Key Words: Anthelmintic activity, Mother tincture, Parasites, Helminthes