The organic compound 2,3,4-trihydroxybenzophenone single crystal was grown by slow evaporation technique. The grown crystal structure was confirmed using powder XRD studies. The melting and decomposition points of the grown crystal were identified using TG/DTA analyses. The kinetic and thermodynamic parameters of the grown crystal were calculated using the Coats-Redfern method. The antibacterial activity of the grown crystals was analyzed for two gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus) and two gram-negative bacterial strains (klebsiella pneumoniae and Escherichia coli) by the Agar Well diffusion method. The grown 2,3,4-trihydroxybenzophenone crystal exhibited powerful antibacterial activities against all the tested bacterial strains in higher concentrations