Aim: To purify urate oxidase from mutated Basillus subtilis.

Methodology: The uric acid lowering protein (urate oxidase) was purified by adopting variouse techniques i.e ammonium sulfate precipitation, ion exchange and gel filtration chromatography.Specific activity, Activity and protein contents in each purification stage were determined.

Results: It was studied that mutated enzyme showed 97.56 U/mg specific activity, 42.93 U/mL activity and 0.44 mg/ml protein contents with 256.73 fold purification. The purified protein was showed molecular weight 34kDa with a single band on SDS-PAGE.

Conclusion: it was concluded that by adopting different purification technique, the enzyme from mutated strain was purified.Aim.

Keywords: urate oxidase (uox), purification, ion exchange chromatography, gel filtration chromatography, uric acid