Pharmacognostic investigation of the fresh, powdered and anatomical sections of the leaves of Otostegia limbata (Benth.) Boiss was carried out to determine its macro- and microscopical characters and also some of its physical constants. The leaves are symmetrical at the base, have an entire edge, an obtuse apex, and a pilose surface. Anisocytic stomata, unicellular, uniseriated-covering trichomes with swelling bases and acute apexes, a prism, clustered calcium oxalate crystals, and fibre components are all present internally. Proteins, starch grains, lignin, mucilage, lipids, and oils were all found by chemo-microscopy. The study's findings may help establish certain diagnostic criteria for the identification of the plant and the creation of a monograph on it.

Key words: pharmacognostic standardization, macro- and microscopical characters, leaf morphology of Otostegia limbata (Benth.) Boiss.