Reactive oxygen species (ROS) can cause hemolysis, resulting in disorders including sickle cell anemia and thalassemia. The relationship between hemolysis and venous thrombosis is complex and encompasses both traditional thrombotic risk factors and unique prothrombotic processes connected to hemolysis. Free radicals produced by autoxidation reactions of sugars and sugar adducts to protein and by autoxidation of unsaturated lipids in plasma and membrane proteins are potential causes of oxidative stress and damage to proteins in diabetes. The present study aimed to evaluate the biological potentials, antidiabetic (α-amylase inhibition assay) antioxidant (DPPH assay), and hemolytic and thrombolytic activity of herbal plant Rosmarinus officinalis. In this research shade dried and powdered plant material was used. Components of Rosmarinus officinalis L. were extracted using methanol by maceration process from which different fractions with different polarity solvents (n-hexane, chloroform, ethyl acetate) were prepared to analyze biological activities. In the current study antidiabetic activity of R. officinalis crude extracts were assessed with different fractions like methanol, n-hexane, chloroform, and ethyl acetate exhibiting higher percentage of inhibition are 76.27± 0.031, 69.91± 0.027, 75± 0.05, 73.30± 0.041, respectively. DPPH scavenging activity has shown a comparative less percentage of inhibition of different fractions like methanol, n-hexane, chloroform, and ethyl acetate are 71.45± 0.012, 58.18± 0.022, 64.02± 0.015, 58.34± 0.031 at higher concentration of 0.1mg. In thrombolytic activity fractions of methanol, n-hexane, chloroform and ethyl acetate showed the highest percentage of lysis at the concentration of 0.1mg/ml is 55.6±0.03, 63.5± 0.06, 53.2± 0.03, 59.9± 0.02. Still, hemolytic activity showed less percentage of inhibition 17.96± 0.04, 7.784± 0.05, 22.15± 0.05, 6.58± 0.07 respectively at the higher concentration of 0.1mg from all other biological activities. Reported biological activities were analyzed statistically, which showed p ˂0.05 shows the significance of activities. From a future perspective Rosmarinus officinalis L. can be further used for in vivo studies for drug toxicity evaluations.

Keywords: Rosmarinus officinalis L, Antioxidant, Antidiabetic, Anti-hemolytic, Anti-thrombotic