The world is in a combat with pollution and depleting natural resource and there is a need to protect our resources by replacing non-renewable with renewable sources. The main objective of this study is to extract biodiesel from dense growing algae that obstructs the aquatic life and clogs water by its vast growth. In this study, Chlorella vulgaris, a single celled (2 to 10µm diameter); flagellate organism having huge importance in aquaculture and environmental sciences is selected. It is commercial because of its rapid reproduction. Utilizing of accumulated lipids as biodiesel is an incredible alternative to fossil fuels which avoids the dependence of non-renewable resources. The algae have been cultivated in three different media for maximum optimization selected as TAP, N8 and Bg11. This is quantitatively analysed for the amount of proteins and carbohydrates by UV-visible spectrophotometer and was utilised for the extraction of PUFA’s (poly unsaturated fatty acids) and production of biodiesel. Based on the retention time by chromatographic technique the highest percentage of PUFA’S was estimated as 33.23% in Bg11 media. The biodiesel was produced by transesterification where the Bg11 and TAP media showed highest viscosity values.

Keywords: PUFA, Chlorella vulgaris, Transesterification, Biodiesel, Lipid