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In-Vitro Cytotoxic and Antioxidant Activities of Salvadora persica in HepG2 Cells

. Fouzia Latif , Mohammad Saleem, Asim Raza & Aisha Mobashar


Abstract

Hepatic carcinoma (HCC) requires prompt clinical management to prevent morbidity and mortality. Current study was aimed to assess in-vitro cytotoxic, antioxidant and wound-healing effects of Salvadora persica (SP) various extracts, SP aqueous extract (SP-AQ), SP ethanolic extract (SP-E) and SP petroleum ether extract (SP-PE) on HCC cell line HepG2. In addition cytoprotective effect of SP extracts on normal cell line Vero was evaluated. Cytotoxic assays (MTT, LDH, Crystal violet and trypan blue), antioxidative enzymes assays (APOX, GSH, SOD and CAT) were performed on HepG2 and Vero cell line. Wound-healing assay on HepG2 cells was done. Results showed that SP different extracts exerted significant cytotoxic effect with antiproliferative activity and increased antioxidaive enzymes level in HepG2 cells. While SP extracts was observed harmless with non-significant cytotoxic effect in Vero cell line and also showed non-significant increase in antioxidative enzymes level in Vero cells. SP extracts at IC50 values did not heal wound in HepG2 cells. Based on these findings, plant SP could be an effective anticancer agent in hepatic carcinoma.

 Keywords: Hepatic carcinoma, cytotoxicity, antioxidant, In-vitro Technique  

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