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Ameliorative effect of Calcium and Magnesium on growth and physiochemical attributes of Trigonella foenum-greacum L., under Polyethylene Glycol Induced Drought Stress

. Hussan Ara Begum, Syed Hidayat Yar & Asif Khan


Abstract

Drought is one of the most devastating factor that adversely affect the growth and development of plants. The aim of our study to check the exogenously applied calcium (Ca) and magnesium (Mg)’s effect on the growth and development of Trigonella foenum-greacum L, under drought stress conditions, in vitro. The experiment was Randomized Complete Block Design (RCBD), where three replicates for each treatment, one treatment was calcium and magnesium ratio 4 and PEG 0.6MPa, 2nd was calcium and magnesium ratio 4 and PEG 0.2 MPa, 3rd calcium and magnesium ratio 2 and PEG 0.6 MPa, 4th calcium and magnesium ratio 2 and PEG 0.2 MPa, 5th calcium and magnesium ratio 0.18 and PEG 0.6 MPa and last one is calcium and magnesium ratio 0.18 and PEG 0.2MPa. Results showed that growth and the physio hormonal parameters of Trigonella foenum garaecum L. under the PEG induced drought stress in the presence of exogenous Ca and Mg ratios. The study also showed that the Ca and Mg ratios had a positive impact on Trigonella foenum-graecum L. growth parameters i.e. root length, fresh weight, dry weight root moisture content, shoot length, fresh and dry weight, leaf area and primary and secondary metabolites under PEG induced drought stress. On the same way,  different growth parameters like, carbohydrates, protein and chlorophyll contents were not affect in the treatment as compared to control. While, in secondary metabolites due the stress the proline content increases and the rest remain the same to the control. It is concluded that PEG induced stress affect plant’s growth and development.

Key words: Calcium; Drought stress; Magnesium; Metabolites; Polyethylene Glycol

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