The Quercus glauca bark was examined for its phytochemical composition and biological activity, including its potential for antibacterial, cytotoxic, phytotoxic, and antioxidant effects. The methanolic extract of the bark's primary and secondary metabolites, including carbohydrates, phenol, tannins, saponins, terpenoids, alkaloids, phlobatanins, and cardiac glycosides, were identified using phytochemical analysis. However, there was no oil, courmine, glycoside, or glycoside. The bark's methanolic extract significantly inhibited the growth of five different bacterial strains, including Escherichia coli, pseudomonas areuginosa, Klebsiella penumoniae, Acinetobacter baumanni, and Staphylococcus aureus. The bark of the plant had notable antifungal results against all three fungal strains when its methanolic extract was tested against Mycospharella citri, Penicillium, and Trichophyton mentagrophytes. According to antimicrobial tests, the bark of Quercus glauca has the potential to treat a wide range of illnesses and disorders in the future. The Brine shrimp fatality experiment was used to determine the cytotoxicity of bark (BSLA). The plant's bark demonstrated cytotoxic properties. The bark of the plant was tested for phytotoxic activities against lemna minor L. plant may one day be employed as a weedicide. Through a DPPH free radical scavenging assay, the bark's antioxidant capacity was evaluated. Bark from the Quercus glauca tree demonstrated antioxidant potential when extracted with methanol. The plant has a lot of promise and may eventually be utilised for additional beneficial applications. This research can help identify and authenticate plants as well as species that could be employed in the future to create novel treatments.

Keywords: Phytochemicals, Antimicrobial, Cytotoxic, Phytotoxic and Antioxidant activity of the bark of Quercus glauca Thunb.