Berberis lyceum L. is a member of Berberidaceae traditionally used as food and folk medicines for the treatment of various diseases like cancer, diabetes, antioxidant and anti-microbial potential. In present study antimicrobial, antioxidant, analgesic and anti-spasmodic activities were taken under consideration via; in vitro and in vivo approaches. The results showed that the root extract of Berberis lycium L. ethanolic extract possess high amount of flavonoid as compared to phenols. Similarly, different phytochemical constituents like alkaloids, tannins, phlobatannins, flavonoids, carbohydrates, phenols, saponins, proteins and glycosides were found in the crude ethanol extract. The antioxidant activity of Berberis lyceum root was found to be the best in ethanol with percent potential was carried out at concentration of 0.05 mg/mL. The high percent potential of Berberis lyceum was observed in the ethanol extract 59.7% (at concentration of 0.05mg/mL) as compared to methanol (52.9%) and aqueous extract (28.5%). Similarly, different extracts of Berberis lycium was found effective in inhibiting the selected bacterial strains, however, methanol extract was found effective against all selected bacterial strains.  In analgesic activity the highest writhing was count at concentration of 300mg/kg with 72% inhibition, followed by concentration of 200mg/kg showed 57.82%. The anti-spasmodic activity, at 300 mg/kg the charcoal meal transit was high and found effective. It is concluded from the present study that Berberis lycium L. has potential to treat different diseases ranging from anti-oxidant to anti-microbial activities effectively and provide a base for isolation of specific compounds and use it on clinical trials.

Key words: analgesic, spasmodic, beberis, antioxidant, phytochemicals