This study was aimed at isolating and identifying fungi associated with seeds of four cultivars of Berseem by using standard Blotter paper and Rag doll method. Eight fungi viz. Aspergillus niger, Alternaria alternata, Cladosporium herbarum, Curvularia lunata, Drechslera tetramera, Fusarium solani, Penicillium sp. and Colletotrichum capsici were isolated and identified. The most common fungal pathogen reported was Fusarium solani found on seeds of all cultivars. The germination test is done to have an idea about germination percentage as affected by seed born fungi. The germination percentages of four varieties were 92%, 85%, 80% & 70% for Berseem cultivars like Peshawari, Faisalabadi, S1 and Agaiti Berseem. The identified fungal pathogens affected germination by 10-30%. Aspergillus niger, Cladosporium herbarum, Curvularia lunata, Drechslera tetramera Penicillium sp. and Colletotrichum capsici are new records to Berseem crop.

Index Terms- Solvent extracts, Mineral analysis, Nutritional components, Secondary metabolites, Phytochemical analysis