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The construction of BCTV infectious clone to produce symptoms in the tomato plants

. Habiba Naz, Muhammad Azmat Ullah Khan & Abdul Qayyum Rao


Abstract

Curtoviruses is one of the important genus of geminiviridae, transmitted by the leafhopper Circulifer tenellus. Virus belong to Curtoviruses infect dicotyledonous plants and have circular single-strand DNA, a monopartite genome (2.9 kb). To characterize any virus at biological and molecular levels, producing an infectious clone of particular virus is essential. Traditional techniques for constructing infectious clones are laborious and time consuming due to many cloning and extraction steps required. We demonstrate a very effective and simple approach to produce an infectious clone of BCTV using Snapgene software, which significantly reduces all the cloning steps. The efficiency of this method was evaluated by confirming the infectivity of BCTV clones using agroinfection.

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