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Light and Scanning Electron Microscopic Investigation of Selected Taxa of Fabaceae and its Utilization for Taxonomic Classification

. Sumaira Shah, Syed Mukaram Shah, Asad Ullah, Usman Ali, Muhammad Nauman Khan, Sabiha Sultana, Khizar Hayat, Rayan Aziz & Muhammed Çetin


Light and scanning electron microscopy were  conducted to study the leaf anatomical features of fourteen species of family Fabaceae. The plant species were Acacia modesta, Albizia lebbeck, Bauhinia purpurea, Bauhinia variegata, Butea monosperma, Cassia fistula, Cassia javanica, Dalbergia sissoo, Erythrina herbacea, Pongamia pinnata, Prosopis juliflora, Robinia pseudoacacia, Sesbania sesban and Sophora secundiflora. Scanning electron microscopy confirm that the stomata found in studied members are paracytic, tetracytic, anisocytic, and animocytic while the shape of epidermal cells are polygonal, elongated, tetragonal, wavy, and irregular type. Microscopic study shows that the maximum number of stomata were found on abaxail surface than adaxial surface. Paracytic type of stomata were found in majority of species while animocytic stomata found only in Dalbergia sissoo.  Different types of stomata were found on both the surface of Fabaceae members but the stomata type is same on both surface of Butea monosperma, Dalbergia sissoo, Erythrina herbacea, Prosopis juliflora, and Sesbania sesban. Sophora secundiflora have highest number (39) of stomata on lower epidermis while Bauhinia purpurea L. and Bauhinia variegata L. have 5 stomata on lower epidermis. On the upper epidermis of Sesbania sesban have 5.33 number of stomata while 2 stomata were found on upper epidermis of Bauhinia purpurea L., Butea monosperma L., and Dalbergia sissoo Roxb. The Pongamia pinnata have highest (83%) stomatal index while Erythrina herbacea have lowest (10.77%) stomatal index on upper epidermis. In Acacia modesta have high (28.6%) stomatal index and Prosopis juliflora have low (10.32%) stomatal index on lower epidermal surface. The study of these anatomical features provide us a valuable taxonomic information for the identification of problematic taxa. 


Index Terms- Fabaceae, Light Microscopy, SEM, Taxonomic significance, Stomatal index

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