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. Shukaib Anwar, Awais Anwar, Seemab Ashraf, Mehmood ul Hassan, Muhammad Sajid, Malik Faizan Shaukat &Syed Tanveer Shah


Current study was conducted to identify and isolate vital phytochemical constituents of Punica granatum leaf extracts and antimicrobial activity of these extracts during March 2021. The experiment was designed under Complete Randomized Design (CRD). The results revealed that leaf extracts of P. granatum contained higher contents of bioactive compounds like Total Phenolic Compounds (119.47mg GAE/g), Total Antioxidants (1.71 DPPH inhibition), the enzyme Catalase (3.92 mg/g), Superoxide Dismutase (0.0503 µg/g) and Peroxidase (0.88 µg/g). To assess the antimicrobial activity of P. granatum extract of different solvents were applied against micro bacterium Escherichia coli, Pseudomonas aurigonisa and Staphylococcus aureus. According to the findings of the antibacterial assay, the hexane and chloroform extracts of the P. granatum leaves showed inhibitory activity against, S. aureus whereas the E. coli and P. aurigonisa bacteria were resistant against the extract constituents. Hexane leaf extracts zones of inhibition against micro bacterium were 8.6mm, 2mm and 2mm, and chloroform leaf extract had zones of inhibition as 6.3mm, 1.33mm and 1.33 mm against S. aureus, P. aurigonisa and E. coli respectively. While distilled water leaf extract zones of inhibition against Bactria was 4.6mm, 0.23mm against S. aureus and P. aurigonisa, but no inhibition against E. coli was recorded.  The presence of these phytochemicals leads to the conclusion that P. granatum leaves have the potential for formation of new drugs and can be used as herbal medicine for treatments of different diseases and is a good natural candidate to produce drugs against micro bacterium.

Key Words: Extract, Antimicrobial Activity, Total Phenolic Compounds, Micro Bacterium, Chloroform Extracts

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