This study evaluated the safety and effectiveness of a formulated herbal toothpaste compared to artificial ones. It focused on developing and testing a toothpaste made from Olea europaea extract and comparing it to commercial herbal and synthetic toothpastes. The Olea europaea leaf extract was analyzed for phytochemicals using GC/MS, revealing beneficial compounds like phenols, fatty acids, terpenes, flavonoids, carotenoids, and alkaloids. Phenolic compounds were also examined with HPLC. The extract's antioxidant activity was tested and showed high effectiveness with an IC50 value of 5.05 μg/ml, as measured by the DPPH radical scavenging method. The study also involved formulating the toothpaste with Olea europaea leaf extract and evaluating it on parameters such as color, taste, appearance, moisture content, pH, spreadability, foaming power, abrasive particles, homogeneity, and stability. The results were comparable to those of commercial herbal and synthetic toothpastes. Additionally, the formulated toothpaste was tested for antibacterial activity against E. coli and Staphylococcus aureus and showed significant effectiveness against both bacteria, outperforming commercial herbal and synthetic toothpastes.

Index Terms- natural Toothpaste; flow ability; direct compression; Olea europaea Leaf