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Microbial Synthesis of Silver Nanoparticles and its Antimicrobial activity

. Vineetha K, Bharath Raj K C, Harish K H and Prashant Nayak


Abstract

The present research work aims at development of reliable and eco friendly method for synthesis of silver nanoparticles. Recently, microorganisms have been explored as potential biofactory for Bio synthesis of silver nanoparticles. In this study, we used soil samples of marine samples and paddy field as source of bacteria, our research showed that extracellular biosynthesis of metallic silver nanoparticles by the reduction of aqueous Ag+ using soil and sea samples as potential candidates for the rapid synthesis of silver nanoparticles. Silver nanoparticles biosynthesis by reduction of silver ion coming in contact with the cell filtrate was fast as it formed within few minutes. UV-visible spectrum of the aqueous media obtained from the above bacteria containing silver ion showed a peak around 420 nm corresponding to the absorbance of silver nanoparticles. Scanning electron microscopy (SEM) micrographs showed formation of well-dispersed silver nanoparticles in the range of 20–70 nm. Antibacterial activity of silver nanoparticles was comparable to amoxicillin.

Keywords: Silver nanoparticle, nanotechnology research, biological synthesis, Scanning electron microscopy, Antibacterial activity.

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