Wedelia trilobata, commonly known as yellow dot or Singapore daisy and in Hindi as pitabhrunga-raaja and belongs to family Asteraceae. Wedelia trilobata used for medicinal benefits in South China, America, Japan, India and other countries for management of many diseases. In folklore, plant is used to treat snake bite, especially in the Daboia russelli envenomation. This reseach work was carried out to evaluate the venom neutralization ability of Wedelia trilobata against Daboia russelli venom. The Ethanolic extract of plant was subjected first time for anti-snake venom activity. Preliminary phytochemical analysis of Wedelia trilobata indicated the presence of alkaloids, terpenoids, saponin, tannins, and flavonoids. Administration of the plant extract orally at different dose levels (100, 200 and 400 mg/kg) effectively neutralized Daboia russelli venom induced lethality, hemorrhage and necrosis. The above observation confirmed that the plant extract possesses snake venom neutralization capacity.

Keywords: Wedelia trilobata, Daboia russelli, Hemorrhage, Necrosis.