Recently, nanotechnology has emerged as a promising interdisciplinary section dealing with research and development in various fields. The advancements of nanotechnology-based approaches in various commercial segments and their direct effect on human life justify the need for their Ecofriendly ways of synthesis .Nanoparticles are generally described as small particles that measures Around 1 nm to 100 nm in size. The variations in shape, size, surface to volume ratio and Composition of nanosized metal nanoparticle provides them unique physical, chemical, and Biological properties that can be used in medicines, electronics, household devices, agriculture, Cosmetics, and pharmaceutical areas.
Pure NiSe2 Nanoparticles were prepared by green synthesis method. The formed samples were characterized by powder XRD, EDX, SEM and Antimicrobial studies.
XRD can be used to identify single crystals, and to reveal the structure of single crystals.. SEM image shows the surface morphology of pure and NiSe2 nanoparticle. From EDX is an analytical technique used for the elemental analysis or chemical characterization of a sample presence of Ni and Se were confirmed.
Antibacterial activity of NiSe2 nanoparticles was performed by Agar well diffusion method against E.Coli, Pseudomonas aeruginosa, Streptococcus oralis, Staphylococcus aureus and propionibacterium acnes was purchased from MTCC, Chandihar, India. The highest zone of inhibition of NiSe2 nanoparticles synthesized by Citrus Limon extract was found against Streptococcus oralis.This field is used for medicine, due to their high antibacterial activity. The antibacterial activity was assayed by measuring the diameter of the inhibition zone formed around the wells. Gentamicin antibiotic was used as a positive control.
Anti-fungal activity of NiSe2 nanoparticles was performed by Agar well diffusion method against Aspergillus Niger, Aspergillus fumigates and Aspergillus flavus. The anti-fungal activity was assayed by measuring the diameter of the inhibition zone formed around the wells. Amphotericin B was used as a positive control.
Key words: green synthesis, nanoparticles, XRD, , SEM, Anti-Microbial studies, antifungal studies.