Takkar Wildlife Sanctuary is home to diverse flora and fauna. The residents of this wildlife have their culture, lifestyle, beliefs, and traditions, and have used native plants in a variety of ways over many generations. In order to preserve the ethnobotanical information of native populations of the area, an ethnobotanical study was conducted to document the herbal medicinal plants. The field study used questionnaires to interview local informants, aged 50 to 65, who were familiar with the common use of herbal medicinal plants. The data were collected based on plant medicinal uses, local names, botanical names, and plant families and a use-value (UV) of each medicinal plant species were calculated. A total of twenty-two medicinally valuable plant species from sixteen families were identified which have been used to treat a wide range of disorders. Fabaceae was the most common plant family in ethnomedicine with four species, followed by Apocynaceae, Asteraceae, and Zygophyllaceae (two species each). As a result of the ethnobotanical experience, leaves (35%) were the most readily used plant part, followed by whole plant parts and roots (16%), fruit, flowers, and seeds (9% each), bark (4%), branches, and latex (2%). Mode of preparation were infusions (35%), powder (20%), decoctions (9%), tea (6%), ash, smoke, wrap, and gum (3% each). In applications of these plants as medicine, oral (81%) intake was the dominant route of administration. On the use value index, Salvadora oleoides (1.55), Prosopis juliflora (1.45), Calotropis procera (1.41), Capparis decidua (1.36), Aerva javanica (1.32), Acacia nilotica (1.27), Leptadenia pyrotechnica (1.23), and Ziziphus nummularia (1.18) were recorded the most useful in medicines purposes. While the rest of the species had a use value of less than 1.5. This research was carried out to document important ethnobotanical plant species in a study area that has not been previously reported (to our knowledge). Ethnobotanical knowledge is only available to elders of the villages and health practitioners. This traditional knowledge is declining due to a lack of interest from younger generations. There are several threats to these medicinal plant species, including overgrazing, uprooting, erosion, population growth, mining, deforestation, and climate change.

Keywords- Ethnobotany, Medicinal plants, use value, Takkar Wildlife Sanctuary, Khairpur