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Study of Molecular Diagnosis and Analysis of Chikungunya Virus by RT PCR and Serological test

. Kashish Patel and Dr. Prashakha J. Shukla


Chikungunya is caused by an arbovirus and spread by the mosquito Aedesaegypti. Chikungunya is a viral disease with symptoms and pathogenesis that are strikingly similar to dengue fever. Chikungunya is caused by the alpha virus, which is spread by the aedes aegypti mosquito, which is only active during the day.For CHIKV, a real-time RT-PCR assay was developed to detect currently circulating virus strains as well as other genotypes.Its sensitivity was compared to a previously published real-time assay and a standard RT-PCR assay.ELISA was used to identify CHIKV immunoglobulin (Ig) G and M antibodies in serum or blood absorbed on filter paper.

Keywords: Chikungunya, Filter paper; Real time RT PCR, IGM Test.

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